http://precedings.nature.com/users/ce34ad71601abbfc0348dd617dbc94f7/ Documents posted by john rossi Nature Publishing Group en Nature Precedings http://precedings.nature.com/images/header_logo.gif http://precedings.nature.com http://dx.doi.org/10.1038/npre.2007.1299.1 The successful use of small interfering RNAs (siRNAs) for therapeutic purposes requires safe and efficient delivery to specific cells and tissues. Here we demonstrate cell type-specific delivery of anti-HIV siRNAs via fusion to an anti-gp120 aptamer. The envelope glycoprotein is expressed on the surface of HIV-1 infected cells, allowing binding and interalization of the aptamer-siRNA chimeric molecules. We demonstrate that the anti-gp120 aptamer-siRNA chimera is specifically taken up by cells expressing HIV-1 gp120, and the appended siRNA is processed by Dicer, releasing an anti-tat/rev siRNA which in turn inhibits HIV replication. We show for the first time a dual functioning aptamer-siRNA chimera in which both the aptamer and the siRNA portions have potent anti-HIV activities and that gp120 expressed on the surface of HIV infected cells can be used for aptamer mediated delivery of anti-HIV siRNAs. http://dx.doi.org/10.1038/npre.2007.1299.1 Wed, 14 Nov 2007 20:51:10 UTC Novel Cell type-specific aptamer-siRNA delivery system for HIV-1 therapy doi:10.1038/npre.2007.1299.1 2007-11-14 John Rossi Nature Precedings 2007-11-14T20:51:10Z Manuscript Biotechnology Molecular Cell Biology http://creativecommons.org/licenses/by/2.5/