http://precedings.nature.com/users/8f3dd13a7fc846552f260f3f9077c2b7/ Documents posted by Tsung Lang Chiu Nature Publishing Group en Nature Precedings http://precedings.nature.com/images/header_logo.gif http://precedings.nature.com http://precedings.nature.com/documents/3101/version/1 Microglia has been found to diversify its function by cancerous cells or in a cancerous environment, thereby contributing to cancer growth and metastasis. Its immuno-activity, however, can be modulated by interleukin-12. So a strategy was designed using AAV2 carrying IL-12 to activate microglia then to eliminate cancerous cells. The transduction efficacy of AAV was evaluated with AAV2 encoding GFP and IL-12 on cancerous and CNS cells. The bioactivity of microglia modulated by IL-12 was examined and death receptors 4 and 5 were detected on cancerous cells. The effects of IL-12 and AAV2/IL-12 on microglial cytoxoxicity were evaluated too. The results demonstrated human cell line DBTRG, surgical specimen of GBM, and rat astrocyte expressed GFP quite well. Tremendous IL-12 secretion was detected in DBTRG, RG2, and astrocyte after transfection of AAV2/IL-12. TRAIL releasing and phagocytotic activity of microglia were significant, increasing (p http://precedings.nature.com/documents/3101/version/1 Fri, 24 Apr 2009 00:10:52 UTC Enhanced anti-cancer activity of microglia by AAV2-mediated IL-12 in the therapy of glioblastoma multiforme hdl:10101/npre.2009.3101.1 2009-04-24 Tsung-Lang Chiu Nature Precedings 2009-04-24T00:10:52Z Manuscript Cancer http://creativecommons.org/licenses/by/3.0/