http://precedings.nature.com/tags/siRNA Recently posted documents tagged with 'siRNA' Nature Publishing Group en Nature Precedings http://precedings.nature.com/images/header_logo.gif http://precedings.nature.com http://dx.doi.org/10.1038/npre.2007.1299.1 The successful use of small interfering RNAs (siRNAs) for therapeutic purposes requires safe and efficient delivery to specific cells and tissues. Here we demonstrate cell type-specific delivery of anti-HIV siRNAs via fusion to an anti-gp120 aptamer. The envelope glycoprotein is expressed on the surface of HIV-1 infected cells, allowing binding and interalization of the aptamer-siRNA chimeric molecules. We demonstrate that the anti-gp120 aptamer-siRNA chimera is specifically taken up by cells expressing HIV-1 gp120, and the appended siRNA is processed by Dicer, releasing an anti-tat/rev siRNA which in turn inhibits HIV replication. We show for the first time a dual functioning aptamer-siRNA chimera in which both the aptamer and the siRNA portions have potent anti-HIV activities and that gp120 expressed on the surface of HIV infected cells can be used for aptamer mediated delivery of anti-HIV siRNAs. http://dx.doi.org/10.1038/npre.2007.1299.1 Wed, 14 Nov 2007 20:51:10 UTC Novel Cell type-specific aptamer-siRNA delivery system for HIV-1 therapy doi:10.1038/npre.2007.1299.1 2007-11-14 John Rossi Nature Precedings 2007-11-14T20:51:10Z Manuscript Biotechnology Molecular Cell Biology http://creativecommons.org/licenses/by/2.5/ http://dx.doi.org/10.1038/npre.2007.1206.1 RNA interference (RNAi) is a conserved biological response to double-stranded RNAs that results in posttranscriptional silencing of target gene expression. Double-stranded processed RNAs of 21-23 nt were found to affect in a non-specific manner a number of signaling and transcription pathways, including interferon and PKR cascades. We report here that siRNAs induce the expression of general transcription factor TRF3, which can trigger an interferon response in mammalian cells. http://dx.doi.org/10.1038/npre.2007.1206.1 Thu, 04 Oct 2007 08:32:42 UTC Activation of TRF3 Transcription Factor by Small Interfering RNAs doi:10.1038/npre.2007.1206.1 2008-07-01 Stephan Persengiev Nature Precedings 2007-10-04T08:32:42Z Manuscript Molecular Cell Biology http://creativecommons.org/licenses/by/2.5/