http://precedings.nature.com/tags/metabolites Recently posted documents tagged with 'metabolites' Nature Publishing Group en Nature Precedings http://precedings.nature.com/images/header_logo.gif http://precedings.nature.com http://precedings.nature.com/documents/3812/version/1 The detection and quantitation of exogenously administered biological macromolecules (e.g. vaccines, peptide and protein therapeutics) and their metabolites is frequently complicated by the presence of a complex endogenous mixture of closely related compounds. We describe a method that incorporates stable isotope labeling of the compound of interest allowing the selective screening of the intact molecule and all metabolites using a modified precursor ion scan. This method involves monitoring the low molecular weight fragment ions produced during MS/MS that distinguish isotopically labelled material from related endogenous compounds. All isotopically labelled substances can be selected using this scanning technique for further analysis whilst other unlabelled and irrelevant substances are ignored. The potential for this technique to be used in metabolism and pharmacokinetic experiments is discussed with specific examples looking at the metabolism of α-synuclein in serum and the brain. http://precedings.nature.com/documents/3812/version/1 Tue, 29 Sep 2009 09:11:02 UTC A novel strategy for the targeted analysis of protein and peptide metabolites hdl:10101/npre.2009.3812.1 2009-09-29 Anthony W. Purcell Nature Precedings 2009-09-29T09:11:02Z Manuscript Biotechnology Immunology Pharmacology http://creativecommons.org/licenses/by/3.0/ http://dx.doi.org/10.1038/npre.2007.1263.2 Background: The ecological role of actinomycetes and their secondary metabolites is not yet completely understood. Hypothesis: Actinomycetes and Streptomyces sp. in particular, are non-obligate predators of bacteria in soil. Evidence: Ability to grow on live bacterial cells as a sole source of nutrients. Prey cell lysis accompanying growth. Circumstantial evidence for the involvement of antimicrobials along with enzymes.Implications: This finding may open up a new source of novel secondary metabolites from the genus. http://dx.doi.org/10.1038/npre.2007.1263.2 Thu, 01 Nov 2007 17:00:03 UTC Streptomyces sp. as predators of bacteria doi:10.1038/npre.2007.1263.2 2007-11-01 Milind G. Watve Nature Precedings 2007-11-01T17:00:03Z Presentation Biotechnology Ecology Microbiology http://creativecommons.org/licenses/by/2.5/ http://dx.doi.org/10.1038/npre.2007.1263.1 Background: The ecological role of actinomycetes and their secondary metabolites is not yet completely understood. Hypothesis: Actinomycetes and Streptomyces sp. in particular, are non-obligate predators of bacteria in soil. Evidence: Ability to grow on live bacterial cells as a sole source of nutrients. Prey cell lysis accompanying growth. Circumstantial evidence for the involvement of antimicrobials along with enzymes.Implications: This finding may open up a new source of novel secondary metabolites from the genus. http://dx.doi.org/10.1038/npre.2007.1263.1 Mon, 29 Oct 2007 10:42:34 UTC Streptomyces sp. as predators of bacteria doi:10.1038/npre.2007.1263.1 2007-10-29 Milind G. Watve Nature Precedings 2007-10-29T10:42:34Z Presentation Biotechnology Ecology Microbiology http://creativecommons.org/licenses/by/2.5/