http://precedings.nature.com/tags/immunology Recently posted documents tagged with 'immunology' Nature Publishing Group en Nature Precedings http://precedings.nature.com/images/header_logo.gif http://precedings.nature.com http://dx.doi.org/10.1038/npre.2009.3159.1 The cDNA sequences of immunoglobulins (IG) and T cell receptors (TR) represent more than one half of the sequences in the IMGT® nucleotide database IMGT/LIGM-DB1 and 75% of them are from human and mouse. A few cDNA are germline but the great majority results from a V-D-J or V-J gene rearrangement, spliced to a C gene. The IG and TR genes have been studied extensively in IMGT® (http://www.imgt.org) 2, which allowed to set up their nomenclature and the corresponding germline reference sequences. These standardized reference directory sets (one for each group of each locus) and the IMGT-ONTOLOGY axioms and derived concepts3 are the key elements indispensable to perform the annotation of IG and TR cDNA sequences. A Java program, IMGT/Automat4, was developed by IMGT®, to automatically annotate the IG and TR cDNA sequences and to produce a totally automatic and complete annotation. More than 9,000 human and mouse cDNA have already been successfully automatically annotated. The quality of the cDNA automatic annotation is equivalent to the quality of the annotation achieved by a human expert. The IMGT® strategy is currently the only way, in the field of immunogenetics, to guarantee the annotation quality and the management of an always increasing number of IG and TR cDNA nucleotide sequences. http://dx.doi.org/10.1038/npre.2009.3159.1 Thu, 23 Apr 2009 17:12:56 UTC IMGT/Automat: the strategy for the annotation of human and mouse cDNA nucleotide sequences of IG and TR doi:10.1038/npre.2009.3159.1 2009-04-23 Géraldine Folch Nature Precedings 2009-04-23T17:12:56Z Poster Genetics & Genomics Immunology Bioinformatics http://creativecommons.org/licenses/by/3.0/ http://precedings.nature.com/documents/2985/version/1 Streptococcus equi subspecies equi (S. equi) is a clonal, equine host-adapted pathogen of global importance that causes a highly contagious suppurative lymphodendopathy of the head and neck, more commonly known as Strangles. The disease is highly prevalent, can be severe and spread easily by visibly infected animals or by carrier animals that show no clinical signs of disease. Antibiotic treatment is usually ineffective. However, the majority of horses develop immunity to re-infection, suggesting that vaccination should be a feasible way to prevent the infection. Live attenuated vaccine strains of S. equi are available but adverse reactions have been reported and they suffer from a short duration of immunity. Thus, a safe and effective vaccine against S. equi is highly desirable. In this report, Welsh mountain ponies vaccinated with a combination of seven recombinant S. equi proteins, were significantly protected from experimental infection by S. equi, resembling the spontaneous disease. The protective antigens consisted of five surface localized proteins and two IgG endopeptidases. The results from a second vaccination trial indicate that the endopeptidases were important for good protection. The similarity of S. equi to other pyogenic streptococci suggests that our findings have broader implications for the prevention of streptococcal infections. http://precedings.nature.com/documents/2985/version/1 Thu, 26 Mar 2009 17:23:17 UTC Protective vaccination in the horse against Streptococcus equi with recombinant antigens hdl:10101/npre.2009.2985.1 2009-03-26 Jan-Ingmar Flock Nature Precedings 2009-03-26T17:23:17Z Manuscript Immunology Microbiology http://creativecommons.org/licenses/by/3.0/ http://precedings.nature.com/documents/1855/version/2 Investigation and experimental design of the study was basically aimed at developing insight into the antigenicity of spermatozoa-associated proteins. Apart from studying the natural antigenicity of washed whole spermatozoa, their immunogenicity was also demonstrated in vitro. The whole live spermatozoa were immobilized and agglutinated in vitro by the antibodies they induced in the laboratory model – a female rabbit. A regular immunization routine induced a high titre of antisperm polyclonal antibodies. To prepare a spermatozoa specific antigen which will not produce a cross-reacting antibody against other human tissues, only the motile and live spermatozoa were selected for antigen preparation. In investigation the laboratory-bred female rabbits were used as the bioactive system of production of antisperm antibody. Agglutination of whole spermatozoa has been observed on slides. The technique though simple is highly eloquent; clumping of spermatozoa confirms the existence of antisperm antibodies in the serum under examination. The results show that nature and pattern of immobilization of active motile spermatozoa are different as observed in different graphs of immobilization. The variation in spermatozoal population in antigen distribution on individual spermatozoa is reflected in different patterns of agglutination. http://precedings.nature.com/documents/1855/version/2 Wed, 25 Jun 2008 16:18:32 UTC Immunogenicity of human spermatozoa hdl:10101/npre.2008.1855.2 2008-06-25 Jhinuk Chatterjee Nature Precedings 2008-06-25T16:18:32Z Manuscript Immunology http://creativecommons.org/licenses/by/3.0/ http://precedings.nature.com/documents/2005/version/1 Current thinking emphasizes the primacy of CD14 in facilitating TLR recognition of microbes to initiate proinflammatory signaling events and the importance of p38-MAPK in augmenting such responses. Herein, this paradigm is challenged by demonstrating that recognition of Borrelia burgdorferi not only triggers an inflammatory response in the absence of CD14, but one that is uncontrolled as a consequence of impaired PI3K/AKT/p38-MAPK signaling and negative regulation of TLR2. CD14 deficiency results in hyperphosphorylation of AKT and reduced activation of p38. Such aberrant signaling leads to decreased negative regulation by SOCS1, SOCS3, and CIS thereby engendering a more severe and persistent inflammatory response to B. burgdorferi. Perturbation of this CD14/p38-MAPK-dependent mechanism of immune regulation may underlie development of infectious chronic inflammatory syndromes. http://precedings.nature.com/documents/2005/version/1 Wed, 25 Jun 2008 10:07:23 UTC CD14 Modulates PI3K/AKT/p38-MAPK Licensing of Negative Regulators of TLR Signaling to Restrain Chronic Inflammation hdl:10101/npre.2008.2005.1 2008-07-01 Timothy J. Sellati Nature Precedings 2008-06-25T10:07:23Z Manuscript Immunology Microbiology http://creativecommons.org/licenses/by/3.0/ http://precedings.nature.com/documents/1855/version/1 Investigation and experimental design of the study was basically aimed at developing insight into the antigenicity of spermatozoa-associated proteins. Apart from studying the natural antigenicity of washed whole spermatozoa, their immunogenicity was also demonstrated in vitro. The whole live spermatozoa were immobilized and agglutinated in vitro by the antibodies they induced in the laboratory model – a female rabbit. A regular immunization routine induced a high titre of antisperm polyclonal antibodies. To prepare a spermatozoa specific antigen which will not produce a cross-reacting antibody against other human tissues, only the motile and live spermatozoa were selected for antigen preparation. In investigation the laboratory-bred female rabbits were used as the bioactive system of production of antisperm antibody. Agglutination of whole spermatozoa has been observed on slides. The technique though simple is highly eloquent; clumping of spermatozoa confirms the existence of antisperm antibodies in the serum under examination. The results show that nature and pattern of immobilization of active motile spermatozoa are different as observed in different graphs of immobilization. The variation in spermatozoal population in antigen distribution on individual spermatozoa is reflected in different patterns of agglutination. http://precedings.nature.com/documents/1855/version/1 Mon, 05 May 2008 16:13:56 UTC Immunogenicity of human spermatozoa hdl:10101/npre.2008.1855.1 2008-05-05 Jhinuk Chatterjee Nature Precedings 2008-05-05T16:13:56Z Manuscript Immunology http://creativecommons.org/licenses/by/3.0/