http://precedings.nature.com/tags/antibiotics Recently posted documents tagged with 'antibiotics' Nature Publishing Group en Nature Precedings http://precedings.nature.com/images/header_logo.gif http://precedings.nature.com http://precedings.nature.com/documents/3068/version/2 WithdrawnIncomplete data http://precedings.nature.com/documents/3068/version/2 Mon, 04 May 2009 02:08:49 UTC Design of selective peptide antibiotics by using the sequence moment concept hdl:10101/npre.2009.3068.2 2009-05-04 Davor Juretic Nature Precedings 2009-05-04T02:08:49Z Manuscript Biotechnology Pharmacology Bioinformatics http://creativecommons.org/licenses/by/3.0/ http://precedings.nature.com/documents/3068/version/1 New antibiotics against multidrug-resistant bacteria are urgently needed, but rapid acquisition of resistance limits their usefulness. Endogenous antimicrobial peptides (AMPs) with moderate selectivity, but multimodal mechanism of action, have remained effective against bacteria for millions of years. Their therapeutic application, however, requires optimizing the balance between antibacterial activity and selectivity, so that rational design methods for increasing selectivity are highly desirable. We have created training (n=36) and testing (n=37) sets from frog-derived AMPs with determined therapeutic index (TI). The 'sequence moments' concept then enabled us to find a one-parameter linear model resulting in a good correlation between measured and predicted TI (r<sup>2</sup>=0.83 and 0.64 for each set, respectively). The concept was then used in the AMP-Designer algorithm to propose primary structures for highly selective AMPs against Gram-negative bacteria. Testing the activity of one such peptide produced a TI>200 as compared to the best AMP in the data-base, with TI=125. http://precedings.nature.com/documents/3068/version/1 Fri, 17 Apr 2009 20:54:43 UTC Design of selective peptide antibiotics by using the sequence moment concept hdl:10101/npre.2009.3068.1 2009-04-17 Davor Juretic Nature Precedings 2009-04-17T20:54:43Z Manuscript Biotechnology Pharmacology Bioinformatics http://creativecommons.org/licenses/by/3.0/ http://precedings.nature.com/documents/1520/version/1 Peptide deformylase (PDF) is a metalloproteinase and executes an essential step in the maturation of proteins in eubacteria, by removing the formyl group from the N-terminal methionine residue of ribosome-synthesized polypeptides. This process is crucial for bacterial survival because mature proteins do not retain N-formyl-methionine, and all known N-terminal peptidases cannot utilize formylated peptides as substrate. Thus, inhibition of PDF is essential to obstruct the bacterial protein maturation process. Antibiotics based on PDF inhibition have the potential to provide the much needed antibacterial activity against most of the major drug-resistant pathogens. This study comprises an implementation of in-silico techniques to validate and map the features of the respective active site of PDF from Leptospira interrogans. Our analysis consolidates PDF as a promising target for developing novel alternatives as well as indicates superior affinity of current therapeutic agents towards it. This consequently provides a new insight for leptospirosis treatment. http://precedings.nature.com/documents/1520/version/1 Mon, 21 Jan 2008 17:30:50 UTC In Silico Docking Analysis of Peptide Deformylase (PDF) – A Novel Target for Prophylaxis of Leptospirosis hdl:10101/npre.2008.1520.1 2008-07-06 Virupaksha A. Bastikar Nature Precedings 2008-01-21T17:30:50Z Manuscript Microbiology Pharmacology Bioinformatics http://creativecommons.org/licenses/by/3.0/ http://dx.doi.org/10.1038/npre.2007.1263.2 Background: The ecological role of actinomycetes and their secondary metabolites is not yet completely understood. Hypothesis: Actinomycetes and Streptomyces sp. in particular, are non-obligate predators of bacteria in soil. Evidence: Ability to grow on live bacterial cells as a sole source of nutrients. Prey cell lysis accompanying growth. Circumstantial evidence for the involvement of antimicrobials along with enzymes.Implications: This finding may open up a new source of novel secondary metabolites from the genus. http://dx.doi.org/10.1038/npre.2007.1263.2 Thu, 01 Nov 2007 17:00:03 UTC Streptomyces sp. as predators of bacteria doi:10.1038/npre.2007.1263.2 2007-11-01 Milind G. Watve Nature Precedings 2007-11-01T17:00:03Z Presentation Biotechnology Ecology Microbiology http://creativecommons.org/licenses/by/2.5/ http://dx.doi.org/10.1038/npre.2007.1263.1 Background: The ecological role of actinomycetes and their secondary metabolites is not yet completely understood. Hypothesis: Actinomycetes and Streptomyces sp. in particular, are non-obligate predators of bacteria in soil. Evidence: Ability to grow on live bacterial cells as a sole source of nutrients. Prey cell lysis accompanying growth. Circumstantial evidence for the involvement of antimicrobials along with enzymes.Implications: This finding may open up a new source of novel secondary metabolites from the genus. http://dx.doi.org/10.1038/npre.2007.1263.1 Mon, 29 Oct 2007 10:42:34 UTC Streptomyces sp. as predators of bacteria doi:10.1038/npre.2007.1263.1 2007-10-29 Milind G. Watve Nature Precedings 2007-10-29T10:42:34Z Presentation Biotechnology Ecology Microbiology http://creativecommons.org/licenses/by/2.5/