Document information

1 vote

Opposite effects of two zinc(II) dithiocarbamates on NF-kB pathway

Boris Cvek¹, Zdenek Dvorak¹, Jan Taraba², Lukas Muller³, Radim Vrzal¹, & Jitka Ulrichova¹

Correspondence: (Login to view email address)

1. Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic
2. Department of Chemistry, Faculty of Science, Masaryk University, Brno, Czech Republic
3. Department of Analytical Chemistry, Faculty of Science, Palacky University, Olomouc, Czech Republic

PPT (878 KB) PDF (727.5 KB)

Document Type:

Poster

Date:

Received 15 August 2007 11:21 UTC; Posted 16 August 2007

Subjects:

Cancer, Chemistry, Molecular Cell Biology, Pharmacology

Tags:

• NF-kappaB
• proteasome
• dithiocarbamate
• AP-1
• cancer
• disulfiram

Abstract:

Inhibiting nuclear factor-kappaB (NF-kB) activation in anticancer and antiinflammatory therapy is of topical interest. Current research in molecular biology has dramatically advanced in the understanding of the cellular events involved in NF-kB induction. Dithiocarbamates, in particular diethyldithiocarbamate and pyrrolidinedithiocarbamate, have been known and widely used as strong inhibitors of NF-kB signaling pathway for more than ten years. Their activity is frequently thought to be due to chelating of zinc or copper present in serum supplemented in the culture medium. Zinc(II) diethyldithiocarbamate (Et2Zn) and zinc(II) dibenzyldithiocarbamate (Bz2Zn) were prepared by direct synthesis in aqueous millieu. They were structurally characterized by X-ray analysis (solid phase) and mass spectrometry (aqueous conditions). Et2Zn and Bz2Zn both in 20 micromolar concentration were applied to HeLa cells. The status of NF-kB signaling was assessed as nuclear translocation of p65 subunit. Surprisingly, Et2Zn activated NF-kB pathway, while TNF-dependent activation of NF-kB was inhibited by Bz2Zn. Our results are preliminary.

Presented at:

VII. interdisciplinary meeting of young biologists, chemists and biochemists, Devet skal, Czech Republic, 12 June 2007

Discussion

Votes:

1 vote

(Login to vote)

Comments:

0 comments

(Login to post a comment)

Share:

(Login to share with a colleague)

Additional information

License:

This document is licensed to the public under the Creative Commons Attribution 2.5 License

How to cite this document:

Cvek, Boris, Dvorak, Zdenek, Taraba, Jan, Muller, Lukas, Vrzal, Radim, and Ulrichova, Jitka. Opposite effects of two zinc(II) dithiocarbamates on NF-kB pathway. Available from Nature Precedings <http://dx.doi.org/10.1038/npre.2007.735.1> (2007)

Version info:

Other versions of this document in Nature Precedings

None.

Other versions of this document elsewhere on the web

None known.

Submit a new version link