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MicroDNAs and Transcriptional Regulation

Reza sheikhnejad¹

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1. TODACO Research and Development, molecular biology

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Document Type:

Manuscript

Date:

Received 04 November 2009 08:33 UTC; Posted 04 November 2009

Subjects:

Cancer, Genetics & Genomics, Molecular Cell Biology

Tags:

• microDNAs
• transcriptional regulation
• apoptosis
• ki-ras
• bcl-2
• Lymphoma
• leukemia

Abstract:

Synthetic "microDNAs (MIDs)"is a new class of ~ 20-25 nucleotide-long DNAs capable of repressing the activity of the target gene at the level of transcription by mechanisms that have not been clarified yet. However they are designed to target non-coding regions of the cancer causing genes, thus interfering with transcription. The inhibition might be possible through the direct binding of MIDs to cis-regulatory sites and/or to some Transcription Factors (TF) that normally activate transcription. Synthetic MIDs in some ways are similar to the newly discovered microRNAs a mechanism by which cell regulates its genetic activities at post-transcriptional level. Synthetic MIDs can provide a powerful tool to prevent massive production of mRNA by undesired gene activities. Therefore drugs are not required to interact with overwhelming number of mRNA and microRNA copies that may present unwanted side effects. In vitro studies suggest that the inhibition of the target gene starts after the first round of DNA replication, usually 24 hours after treatment depending on cell doubling time. Cell growth suppression maximizes at day 6 or 7 and the inhibition effect is sustained for weeks. We have shown that blocking of both bcl-2 and k-ras transcription by their specific microDNA Inhibitors induced apoptosis in HL60 leukemia cells and B-cell lymphomas.

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License:

This document is licensed to the public under the Creative Commons Attribution 3.0 License

How to cite this document:

sheikhnejad, Reza. MicroDNAs and Transcriptional Regulation. Available from Nature Precedings <http://hdl.handle.net/10101/npre.2009.3931.1> (2009)

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