Comparison of Plasmid yield between L.B. and using Molasses as a medium
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- RESEARCH SCHOLAR, MICROBIOLOGY
- professer, Microbiology
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- Manuscript
- Date:
- Received 24 October 2009 13:28 UTC; Posted 28 October 2009
- Subjects:
- Biotechnology
- Abstract:
During the initial studies I have found that the molasses may be a good alternative to costly L.B media where one can manipulate the C/N ratio as per requirement of cell. Molasses is one of the best alternatives as it is cheap and can be easily manipulated. Escherichia coli is a bacterium that is commonly found in the lower intestine of warm-blooded animals. it is possible to produce vaccine by molasses medium. The paper is opening a new face of study.
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Sharma, Nitin and Kaushik, Purshottum. Comparison of Plasmid yield between L.B. and using Molasses as a medium. Available from Nature Precedings <http://dx.doi.org/10.1038/npre.2009.3897.1> (2009)
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Nitin Sharma on 24 October 2009 13:33 UTC
The above experiment shows that it is possible to produce vaccine in molasses. Molasses is one of the best alternatives as it is cheap and can be easily manipulated. The Carbon: Nitrogen ratio of molasses can be easily manipulated. This particular technology holds the key for the future, as it reduce the cost of production of DNA vaccines and making it available to everyone. Further study ahead it is possible to use this technology for production of vaccine for human uses.