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Electron microscopy analysis of FcRγ localization after its capture by T cells by trogocytosis

Stephanie Balor¹, Marc Daëron², Pierre Bruhns² & Denis Hudrisier³

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1. Université de Toulouse, Toulouse, France
2. Institut Pasteur, Département d’Immunologie, Unité d’Allergologie Moléculaire et Cellulaire, Paris and INSERM U760, Paris, France
3. IPBS, CNRS, Toulouse and Université de Toulouse, France

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Date:

Received 02 October 2009 14:08 UTC; Posted 05 October 2009

Subjects:

Immunology

Tags:

• FcR
• trogocytosis
• t cell

Abstract:

T cells acquire various proteins from their cellular partners by the process of trogocytosis. We recently demonstrated that the FcγRIIIA receptor and its associated FcRγ are captured by T cells during their co-culture with FcγR-expressing target cells upon both antigen- or antibody-mediated stimulation. Interestingly, we found that FcR captured by T cells could bind ligands but did not transmit detectable intracellular signals or signaling-depending functions upon ligand binding suggesting their improper integration in the recipient T cell membrane. In this study, we provide morphological data in support of this hypothesis. Indeed, we show that the FcRγ-subunit, which we used as a fusion to GFP, was clearly present at the plasma membrane of donor cells, but was detected within structures that were in close contact of, but apparently not integrated in, the plasma membrane of recipient T cells.

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This document is licensed to the public under the Creative Commons Attribution 3.0 License

How to cite this document:

Balor, Stephanie, Daëron, Marc, Bruhns, Pierre, and Hudrisier, Denis. Electron microscopy analysis of FcRγ localization after its capture by T cells by trogocytosis. Available from Nature Precedings <http://dx.doi.org/10.1038/npre.2009.3824.1> (2009)

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