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A novel strategy for the targeted analysis of protein and peptide metabolites

Nicholas A. Williamson¹, Charles Reilly¹, Chor-Teck Tan¹, Sri-Harsha Ramarathinam¹, Alun Jones², Christie L. Hunter³, Francis R. Rooney³ & Anthony W. Purcell¹

Correspondence: (Login to view email address)

1. University of Melbourne, Biochemistry and Molecular Biology
2. University of Queensland, Institute for Molecular Bioscience
3. AB SCIEX

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Document Type:

Manuscript

Date:

Received 29 September 2009 07:56 UTC; Posted 29 September 2009

Subjects:

Biotechnology, Immunology, Pharmacology

Tags:

• proteomics
• Biochemistry
• metabolites

Abstract:

The detection and quantitation of exogenously administered biological macromolecules (e.g. vaccines, peptide and protein therapeutics) and their metabolites is frequently complicated by the presence of a complex endogenous mixture of closely related compounds. We describe a method that incorporates stable isotope labeling of the compound of interest allowing the selective screening of the intact molecule and all metabolites using a modified precursor ion scan. This method involves monitoring the low molecular weight fragment ions produced during MS/MS that distinguish isotopically labelled material from related endogenous compounds. All isotopically labelled substances can be selected using this scanning technique for further analysis whilst other unlabelled and irrelevant substances are ignored. The potential for this technique to be used in metabolism and pharmacokinetic experiments is discussed with specific examples looking at the metabolism of α-synuclein in serum and the brain.

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This document is licensed to the public under the Creative Commons Attribution 3.0 License

How to cite this document:

Williamson, Nicholas, Reilly, Charles, Tan, Chor-Teck, Ramarathinam, Sri-Harsha, Jones, Alun, Hunter, Christie, Rooney, Francis, and Purcell, Anthony. A novel strategy for the targeted analysis of protein and peptide metabolites. Available from Nature Precedings <http://hdl.handle.net/10101/npre.2009.3812.1> (2009)

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