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Preconditioning of Nanofibers with Embryonic Stem Cells Is Nephroprotective

Yin Wang¹, Erica Bakota², Hon-Chiu Leung³, Mark Entman⁴, Sung Jung⁵, Ana Crane⁶, Jeffrey Hartgerink⁷ & Farhad Danesh⁸

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1. Baylor College of Medicine, Medicine
2. Rice University, Chemistry
3. Baylor College of Medicine, Pediatrics-Hema & Oncology
4. Baylor College of Medicine, cardiovascular Sciences
5. Baylor College of Medicine, Biochemistry
6. Baylor College of Medicine, Center for Cell and Gene Therapy
7. Rice University, Chemistry and Biochemical Engineering
8. Baylor College of Medicne, Medicine/Nephrology

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Document Type:

Manuscript

Date:

Received 26 August 2009 19:07 UTC; Posted 27 August 2009

Subjects:

Biotechnology, Pharmacology

Tags:

• nanofibril
• kidney disease
• stem cells

Abstract:

We report that "preconditioning" of self-assembling nanofibers with embryonic stem cells (ESC) can be used to effectively deliver paracrine/endocrine factors from ESCs to the target tissues. By designing a novel self-assembled peptide nanofiber hydrogel, we examined the regenerative effect of bioactive molecules secreted from ESC in acute kidney injury (AKI). Our findings indicate that preconditioning of nanofibers with ESCs effectively delivers bioactive molecules from ESCs in vitro and in vivo. Our data show that preconditioned nanofibers reverse LPS-induced cell hyperpermeability and apoptosis in vitro. The in vivo data indicate that preconditioned nanofibers significantly improve LPS-induced AKI. Our results also suggest that the Rho kinase inhibition contributes to the renoprotective effect of preconditioned nanofibers. We propose that the use of preconditioned nanofibers is a novel method of stem cell therapy which provides beneficial effects of stem cells, while circumventing many limitations of stem cell therapy, including the issues of cell engraftment, cell viability, immune tolerance, and formation of teratomas.

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This document is licensed to the public under the Creative Commons Attribution 3.0 License

How to cite this document:

Wang, Yin, Bakota, Erica, Leung, Hon-Chiu, Entman, Mark, Jung, Sung, Crane, Ana, Hartgerink, Jeffrey, and Danesh, Farhad. Preconditioning of Nanofibers with Embryonic Stem Cells Is Nephroprotective. Available from Nature Precedings <http://hdl.handle.net/10101/npre.2009.3692.1> (2009)

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