Inhibition of Acid Phosphatase Enzyme Activity in the Presence of Noncrystalline Calcium Phosphate and Nanocrystalline Calcium Apatite: a Preliminary Study
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- Institute of General & Inorganic Chemistry, Bulgarian Academy of Sciences
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- Document Type:
- Manuscript
- Date:
- Received 20 August 2009 11:52 UTC; Posted 20 August 2009
- Subjects:
- Chemistry, Molecular Cell Biology
- Abstract:
Background
Our bones are remodelled repeatedly during life. A new and “healthy” bone tissue replaces the old one. Accordingly, the bone degrading cells, the osteoclasts, prefer old and fatigued bone. The young bone mineral, i.e. calcium apatite, is less crystalline than the mature one. Is it possible that the osteoclasts distinguish between relatively old and new bone via its crystallinity?Methodology/Principal Findings
Acid phosphatase is an enzyme largely expressed by the osteoclasts during resorption and therefore used as a marker of the cells activity. This study explores whether its enzymatic activity would be decreased in the presence of biomimitetically prepared noncrystalline calcium phosphate and nanocrystalline bone resembling calcium apatite. The results showed that both biomimetic samples decreased significantly the enzyme activity while synthetic calcium phosphate samples did not. Consistent with our hypothesis the noncrystalline calcium phosphate had the greater inhibition effect.Conclusions/Significance
The in vitro data suggests that the bone-resorbing cells activity could be regulated by the degree of crystallinity of the bone mineral. These findings report that changes in the properties of biogenic inorganic materials induce specific interactions with biomolecules which in turn should define the cell behaviour. These results can establish an interesting mechanism of cell regulation if confirmed in vivo.
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- This document is licensed to the public under the Creative Commons Attribution 3.0 License
- How to cite this document:
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Pekounov, Yassen. Inhibition of Acid Phosphatase Enzyme Activity in the Presence of Noncrystalline Calcium Phosphate and Nanocrystalline Calcium Apatite: a Preliminary Study . Available from Nature Precedings <http://hdl.handle.net/10101/npre.2009.3658.1> (2009)
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