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Gender differences in a Drosophila transcriptomic model of chronic pentylenetetrazole induced behavioral deficit

Abhay Sharma¹, Farhan Mohammad² & Priyanka Singh¹

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1. Institute of Genomics and Integrative Biology, Council of Scientific and Industrial Research, Mall Road, Delhi University Campus, Delhi 110007, India
2. Institute of Genomics and Integrative Biology, Council of Scientific and Industrial Research, Mall Road, Delhi University Campus, Delhi 110007, India. Present address: Tata Institute of Fundamental Research, Department of Biological Research, Mumbai-5

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Document Type:

Manuscript

Date:

Received 25 July 2009 08:04 UTC; Posted 27 July 2009

Subjects:

Genetics & Genomics, Neuroscience, Pharmacology, Bioinformatics

Tags:

• Drosophila
• transcriptomic
• gene expression
• microarrays
• pentylenetetrazole
• gender differences
• sex differences
• epileptogenesis

Abstract:

A male Drosophila model of locomotor deficit induced by chronic pentylenetetrazole (PTZ), a proconvulsant used to model epileptogenesis in rodents, has recently been described. Antiepileptic drugs (AEDs) ameliorate development of this behavioral abnormality. Time-series of microarray profiling of heads of male flies treated with PTZ has shown epileptogenesis-like transcriptomic perturbation in the fly model. Gender differences are known to exist in neurological and psychiatric conditions including epileptogenesis. We describe here the effects of chronic PTZ in Drosophila females, and compare the results with the male model. As in males, chronic PTZ was found found to cause a decreased climbing speed in females. In males, overrepresentation of Wnt, MAPK, TGF-beta, JAK-STAT, Cell communication, and Dorso-Ventral axis formation pathways in downregulated genes was previously described. Of these, female genes showed enrichment only for Dorso-Ventral axis formation. Most significant, ribosomal pathway was uniquely overrepresented in genes downregulated in females. Gender differences thus exist in the Drosophila model. Gender neutral, Dorso-Ventral axis formation may be considered as the candidate causal pathway in chronic pentylenetetrazole induced behavioral deficit. Prior evidence of developmental mechanisms in epileptogenesis underscores the usefulness of fly model. Gender specific pathways may provide a lead for understanding brain dimorphism in neuropsychiatric disorders.

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This document is licensed to the public under the Creative Commons Attribution 3.0 License

How to cite this document:

Sharma, Abhay, Mohammad, Farhan, and Singh, Priyanka. Gender differences in a Drosophila transcriptomic model of chronic pentylenetetrazole induced behavioral deficit. Available from Nature Precedings <http://hdl.handle.net/10101/npre.2009.3460.1> (2009)

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