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A novel Drosophila transcriptomic and behavioral model detects potassium chloride with therapeutic potential

Farhan Mohammad¹, Priyanka Singh² & Abhay Sharma²

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1. Institute of Genomics and Integrative Biology, Council of Scientific and Industrial Research, Mall Road, Delhi University Campus, Delhi 110007, India. Present address: Tata Institute of Fundamental Research, Department of Biological Research, Dr. Homi Bha
2. Institute of Genomics and Integrative Biology, Council of Scientific and Industrial Research, Mall Road, Delhi University Campus, Delhi 110007, India.

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Document Type:

Manuscript

Date:

Received 14 July 2009 15:23 UTC; Posted 17 July 2009

Subjects:

Genetics & Genomics, Neuroscience, Pharmacology, Bioinformatics

Tags:

• Potassium chloride
• antieptic drugs
• levetiracetam
• valproate
• ethosuximide
• microarray
• gene expression
• Drosophila

Abstract:

In a newly described Drosophila model, two of the five antiepileptic drugs (AEDs) tested, sodium valproate (NaVP) and levetiracetam (LEV), not ethosuximide (ETH), gabapentin (GBP) and vigabatrin (VGB), ameliorate development of chronic pentylenetetrazole (PTZ) induced locomotor alteration. To further characterize the model, we analyze here the microarray expression profile of heads of flies treated with depolarizing compound potassium chloride (KCl). Surprisingly, microarray clustering showed unexpected similarity among KCl, LEV and NaVP. Further, like the two effective AEDs in the fly model, KCl regulated genes overrepresented ribosomal pathway. Interestingly, KCl also ameliorated development of locomotor deficit in the chronic PTZ model. Both transcriptomic and behavioral analyses thus showed LEV- and NaVP- like neuroprotective effect of KCl. This is consistent with neuroprotective effect of KCl observed previously in mammalian system. The Drosophila model thus provides a unique opportunity to understand long term mechanisms of neuroactive compounds.

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This document is licensed to the public under the Creative Commons Attribution 3.0 License

How to cite this document:

Mohammad, Farhan, Singh, Priyanka, and Sharma, Abhay. A novel Drosophila transcriptomic and behavioral model detects potassium chloride with therapeutic potential. Available from Nature Precedings <http://hdl.handle.net/10101/npre.2009.3429.1> (2009)

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