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Rescue with an anti-inflammatory peptide of chickens infected H5N1 avian flu

Masaki M. Imai¹, Alan A. Okada², Hidechika H. Okada³, Ekowati E. Handharyani⁴, Yasuhiro Tsukamoto⁵, Kazuhide K. Adachi⁵, Noriko N. Okada⁶ & Retno Damajanti R. D. Soejoedono⁴

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1. Nagoya CIty University, Immunology
2. Institute for Protein Science Co.,
3. Institute for Protein Science Co. Ltd., Research Department
4. Bogor Agriculture University, Laboratory of Pathology
5. Kyoto Prefecture University, Laboratory of Animal Hygiene
6. Department of Immunology, Nagoya City Univ Graduate School of Medical Sciences

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Manuscript

Date:

Received 14 July 2009 11:43 UTC; Posted 17 July 2009

Subjects:

Immunology, Microbiology, Pharmacology

Tags:

• ETR-P1/fl rescued all infected chikens

Abstract:

Chickens suffering from avian flu caused by H5N1 influenza virus are destined to die within 2 days due to a systemic inflammatory response. Since HVJ infection (1,2) and influenza virus infection (3,4) cause infected cells to activate homologous serum complement, the systemic inflammatory response elicited could be attributed to the unlimited generation of C5a anaphylatoxin of the complement system, which is a causative peptide of serious inflammation. In monkeys inoculated with a lethal dose of LPS (4 mg/kg body weight), inhibition of C5a by an inhibitory peptide termed AcPepA (5) rescued these animals from serious septic shock which would have resulted in death within a day (6). Therefore, we tested whether AcPepA could also have a beneficial effect on chickens with bird flu. On another front, enhanced production of endothelin-1 (ET-1) and the activation of mast cells (MCs) have been implicated in granulocyte sequestration (7). An endothelin receptor derived antisense homology box peptide (8) designated ETR-P1/fl was shown to antagonize endothelin A receptor (ET-A receptor) (9) and reduce such inflammatory responses as endotoxin-shock (10) and hemorrhagic shock (11), thereby suppressing histamine release in the circulation (12). Thus, we also administered ETR-P1/fl to bird flu chickens expecting suppression of a systemic inflammatory response.

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This document is licensed to the public under the Creative Commons Attribution 3.0 License

How to cite this document:

Imai, Masaki, Okada, Alan, Okada, Hidechika, Handharyani, Ekowati, Tsukamoto, Yasuhiro, Adachi, Kazuhide, Okada, Noriko, and Soejoedono, Retno Damajanti. Rescue with an anti-inflammatory peptide of chickens infected H5N1 avian flu. Available from Nature Precedings <http://hdl.handle.net/10101/npre.2009.3425.1> (2009)

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