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Salmonella typhimurium harboring plasmid expressing interleukin-12 induced attenuation of infection and protective immune responses

Yong Keun Park¹, Yoon Won Suck¹ & Lee Seung Seok¹

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1. Department of Biotechnology, School of Life Sciences and Biotechnology, Korea University, Seoul, Korea

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Date:

Received 09 July 2009 01:50 UTC; Posted 10 July 2009

Subjects:

Biotechnology

Tags:

• IL-12
• Salmonella
• Vaccine
• immune defense

Abstract:

IL-12 is known to be an essential cytokine which appears to provide protective immunity against intracellular bacteria, such as Salmonella. In this study, we investigated the possibility of developing a vaccine using IL-12 against virulent Salmonella. We used the host defense system activated by cytokine IL-12. The highly virulent Salmonella strain (Salmonella typhimurium UK-1) was transformed with cytokine-expressing plasmids. These live, wild-type pathogens were used as vaccine strains without undergoing any other biological or genetic attenuating processes. The newly developed strains induced partial protection from infections (30-40%). Of note, the interleukin-12 transformed pathogen was safe upon immunization with low doses (103 CFU), induced IgG responses, and stimulated protective immune responses against Salmonella Typhimurium in mice (80-100%). These results suggest that IL-12 induced attenuation of wild-type Salmonella in the host infection stage and vaccine development using the wild-type strain harboring IL-12 secreting plasmids may be considered as an alternative process for intracellular bacterial vaccine development without the inconvenience of time-consuming attenuation processes.

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This document is licensed to the public under the Creative Commons Attribution 3.0 License

How to cite this document:

Park, Yong Keun, Won Suck, Yoon, and Seung Seok, Lee. Salmonella typhimurium harboring plasmid expressing interleukin-12 induced attenuation of infection and protective immune responses. Available from Nature Precedings <http://hdl.handle.net/10101/npre.2009.3413.1> (2009)

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