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Development of a new method to preserve caprine cauda epididymal spermatozoa in-situ at -10 degrees Centigrade

Uttam Datta¹, M Chandrasekar¹, Manik Lal Hembram¹ & Raju Dasgupta²

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1. Department of Veterinary Gynaecology & Obstetrics, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, 37 & 68 K. B. Sarani Belgachia, Kolkata -700037, West Bengal, India
2. Department of Animal Genetics and Breeding, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, 37 & 68 K. B. Sarani Belgachia, Kolkata -700037, West Bengal, India

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Document Type:

Manuscript

Date:

Received 26 June 2009 10:45 UTC; Posted 26 June 2009

Subjects:

Biotechnology

Tags:

• Coenzyme Q10
• electrolyte-free medium
• Epididymal spermatozoa
• Freezing
• Hypo-osmotic swelling test

Abstract:

Electrolyte free mediums prepared with soybean lecithin-glycerol,Coenzyme Q10–glycerol and soybean lecithin-Coenzyme Q10-glycerol were inoculated separately into ligated cauda epididymides, equilibrated 2 h at 5 degree centigrade, wrapped with aluminium foils along with testis and freezed at -10 degree centigrade.Spermatozoan characters were evaluated 7 and 21 days after thawing at 38.5 degree centigrade in a water bath for 5 min. Spermatozoan characters were diminished gradually and significantly (P<0.05) between the mediums and observation days. Soybean lecithin-CoenzymeQ10-glycerol effectively protected spermatozoa against cold shock where spermatozoan progressive motility, livability, hypo-osmotic swelling positivity were 30.2 ± 0.62; 45.2 ± 0.82 and 41.6 ± 0.79 percent respectively on day 21. This new method is better than preservation of in-situ epididymal spermatozoa at 5 degree centigrade for a short duration. Therefore, present method can be adopted effectively in field conditions for transportation of freezed epididymides and for re-utilization of maximum functional gametes by artificial reproductive technologies to conserve valuable animals after postmortem / slaughter.

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This document is licensed to the public under the Creative Commons Attribution 3.0 License

How to cite this document:

Datta, Uttam, Chandrasekar, M, Hembram, Manik Lal, and Dasgupta, Raju. Development of a new method to preserve caprine cauda epididymal spermatozoa in-situ at -10 degrees Centigrade. Available from Nature Precedings <http://hdl.handle.net/10101/npre.2009.3377.1> (2009)

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