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Phylogeny of conserved adenines in linkers of Group-I introns

Natarajan Ganesan¹ and Chandrasekhar Kesavan²

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1. Georgetown University, Washington, D.C., USA
2. Musculoskeletal Disease Center, JLP VA Medical Center, CA, USA

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Document Type:

Manuscript

Date:

Received 28 February 2009 19:36 UTC; Posted 03 March 2009

Subjects:

Molecular Cell Biology, Bioinformatics, Evolutionary Biology

Tags:

• RNA
• Group-I intron
• adenine conservation
• riboyzme

Abstract:

We have analyzed the linkers in group-I introns, a characteristic region that is crucial to the folding and splicing process of the folded RNA, in seventy sequences spreading across r-RNA, t-RNA and organelle genes from various organisms including algae, fungi and protozoa. The study revealed a high degree of consensus of specific adenine residues in J3/4, J6/7 and J8/7 stems of the linker regions that were required to stabilize the local orientation, either as single residue or by forming unusual base pairs along with divalent metal ions. Conservation of these residues in the Group-I intron linkers suggests their significant contribution to the folded structure whose bonding and geometry recruit metal ions to interact in stabilizing the folded nature of RNA.

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This document is licensed to the public under the Creative Commons Attribution 3.0 License

How to cite this document:

Ganesan, Natarajan and Kesavan, Chandrasekhar. Phylogeny of conserved adenines in linkers of Group-I introns . Available from Nature Precedings <http://hdl.handle.net/10101/npre.2009.2909.1> (2009)

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