5 votes

Large-scale imaging of brain network activity from >10,000 neocortical cells

Shigehiro Namiki¹, Norio Matsuki² & Yuji Ikegaya²

Correspondence: (Login to view email address)

1. Graduate School of Life and Environmental Sciences, University of Tsukuba
2. Graduate School of Pharmaceutical Sciences, The University of Tokyo

PDF (1.2 MB)

Document Type:

Manuscript

Date:

Received 23 February 2009 03:59 UTC; Posted 23 February 2009

Subjects:

Neuroscience, Bioinformatics

Tags:

• calcium
• Imaging
• network
• action potential
• spike
• neuron
• neocortex
• hippocampus
• large-scale
• microcircuit

Abstract:

Large-scale recording from populations of neurons is a promising strategy in the study of complex brain function. Here we introduce a simple optical technique that simultaneously probes the calcium activity of ~10,000 cells at the single cell resolution in vitro. We employed a combination of a low-magnification objective lens and an electron-multiplying charge-coupled device megapixel camera to achieve large-view-field and high-resolution imaging.

Discussion

Votes:

5 votes

(Login to vote)

Comments:

0 comments

(Login to post a comment)

Share:

(Login to share with a colleague)

Additional information

License:

This document is licensed to the public under the Creative Commons Attribution 3.0 License

How to cite this document:

Namiki, Shigehiro, Matsuki, Norio, and Ikegaya, Yuji . Large-scale imaging of brain network activity from >10,000 neocortical cells. Available from Nature Precedings <http://hdl.handle.net/10101/npre.2009.2893.1> (2009)

Version info:

Other versions of this document in Nature Precedings

None.

Other versions of this document elsewhere on the web

None known.

Submit a new version link