3D model of lamprey estrogen receptor with estradiol and 15α-hydroxy-estradiol
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- University of California, San Diego
- Document Type:
- Manuscript
- Date:
- Received 09 January 2009 02:16 UTC; Posted 09 January 2009
- Subjects:
- Developmental Biology, Ecology, Earth & Environment
- Abstract:
Lamprey, a basal vertebrate, contains orthologs of the estrogen receptor (ER), progesterone receptor and corticoid receptor. A perplexing property of lamprey is that 15α-hydroxy-steroids are active steroids. For example, 15α-hydroxy-estradiol [15α-OH-E2] is the estrogen, instead of estradiol (E2). To investigate how 15α-OH-E2 binds lamprey ER, we constructed a 3D model of the lamprey ER with E2 and 15α-OH-E2. Our 3D model shows that Sδ on Met-409 can form a hydrogen bond with the 15α-hydroxyl on 15α-OH-E2. In human ERα, the corresponding residue Ile-424 has a van der Waals contact with 15α-OH-E2. BLAST analysis of GenBank indicates that among vertebrate ERs, only lamprey ER contains a methionine at this position. Thus, the contact between Sδ on Met-409 and 15α-OH-E2 is unique. Our 3D model of lamprey ER should prove useful in virtual screening of chemical libraries to identify compounds for controlling reproduction in sea lamprey, an environmental pest in the Great Lakes in the USA.
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- License:
- This document is licensed to the public under the Creative Commons Attribution 3.0 License
- How to cite this document:
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Baker, Michael, Chang, David, and Chandsawangbhuwana, Charlie. 3D model of lamprey estrogen receptor with estradiol and 15α-hydroxy-estradiol. Available from Nature Precedings <http://hdl.handle.net/10101/npre.2009.2768.1> (2009)
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