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Gene Transfer of Calcitonin Gene-Related Peptide Inhibits Macrophages and Inflammatory Mediators in Vein Graft Disease

Xiaoning Zhang¹, Jian Zhuang¹, Hongsui Wu¹, Zhihong Chen¹, Jian Su¹, Shiliang Chen¹, Jianguang Chen¹, Yuhui Zheng² & Huanxin Yang²

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1. Guangdong Provincial Cardiovascular Disease Research Institute, Guangdong Provincial People’s Hospital
2. Xiehe Hospital, Fujian Medical University

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Document Type:

Manuscript

Date:

Received 31 October 2008 18:14 UTC; Posted 06 November 2008

Subjects:

Molecular Cell Biology

Tags:

• vein graft disease
• calcitonin gene-related peptide
• gene therapy

Abstract:

Vein graft disease is a chronic inflammatory disease and limits the late results of coronary revascularization. Calcitonin gene-related peptide (CGRP) inhibits macrophages infiltrated and inflammatory mediators, we hypothesized that transfected CGRP gene inhibits macrophages infiltrated and inflammatory mediators in vein graft disease. Autologous rabbit jugular vein grafts were incubated ex vivo in a solution of mosaic adeno-associated virus vectors containing CGRP gene (AAV2/1.CGRP) 、escherichia coli lac Z gene (AAV2/1.LacZ) or saline and then interposed in the carotid artery. Intima/media ratio were evaluated at postoperative 4 weeks, Macrophages were marked with CD68 antibody by immunocytochemistry. Inflammatory mediators were mensurated with real-time PCR. Neointimal thickening was significantly suppressed in AAV2/1.CGRP group. Macrophages infiltrated and inflammatory mediators monocyte chemoattractant protein-1 (MCP-1)、tumor necrosis factorα(TNF-α)、inducible nitricoxide synthase (iNOS)、matrix metalloproteinase-9 (MMP-9) was significantly suppressed in AAV2/1.CGRP group.Gene transfected AAV2/1.CGRP suppressed neointimal hyperplasia in vein graft disease by suppressed macrophages infiltrated and inflammatory mediators.

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This document is licensed to the public under the Creative Commons Attribution 3.0 License

How to cite this document:

Zhang, Xiaoning, Zhuang, Jian, Wu, Hongsui, Chen, Zhihong, Su, Jian, Chen, Shiliang, Chen, Jianguang, Zheng, Yuhui, and Yang, Huanxin. Gene Transfer of Calcitonin Gene-Related Peptide Inhibits Macrophages and Inflammatory Mediators in Vein Graft Disease. Available from Nature Precedings <http://hdl.handle.net/10101/npre.2008.2456.1> (2008)

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