Detection of genetic diversity among Indian strains of Xanthomonas campestris pv. mangiferaeindicae using PCR-RAPD
Correspondence: (Login to view email address)
- Central Institute for Subtropical Horticulture, P.O. Kokori, Rahmankera, Lucknow, India
- Document Type:
- Manuscript
- Date:
- Received 16 October 2008 15:53 UTC; Posted 16 October 2008
- Subjects:
- Biotechnology, Microbiology
- Abstract:
The randomly amplified polymorphic DNA (RAPD) technique was used to investigate the genetic diversity in 6 strains of Xanthomonas campestris pv. mangiferaeindicae (Xcmi), the causal pathogen of mango bacterial canker disease (MBCD). The RAPD analysis was also intended to identify molecular markers, specific to the species to develop PCR-based markers for detection of Xcmi in mango field and planting materials. Twenty RAPD primers (CP 1-CP 20) were evaluated to establish molecular characters and genetic variability in the genome of Xcmi. Among these, only 4 were found efficient for development of reproducible banding pattern. It has been observed that the largest and smallest amplified RAPD products were of 2.036 and 0.201 kbp. A total of 136 bands were scored against 6 strains of Xcmi. There was 7.66 per cent polymorphism in individual isolates which indicates significant polymorphism among the evaluated strains, with mean difference of 0.33 (Xcmi 2 vs. Xcmi 8) and 0.29 (Xcmi 10 vs. Xcmi 12). However, the single linkage euclidean distances were statistically significant (P>0.05), i.e., 0.58. The markers CP 5, 10, 16 and 19 were amplified in all the strains with polymorphic alleles, which indicates that these markers could be used for rapid detection of genetic variability in Xcmi strains.
Discussion
- Votes:
-
4 votes
- Comments:
-
2 comments
MBCD is current thurst area of research for mango cultivars specially in a country like India. So paper is good enough to designate the variability among selected representative isolates of MBCD pathogen in India.
- (Login to share with a colleague)
Additional information
- License:
- This document is licensed to the public under the Creative Commons Attribution 3.0 License
- How to cite this document:
-
Kishun, Ram and Gupta, Vijai Kumar. Detection of genetic diversity among Indian strains of Xanthomonas campestris pv. mangiferaeindicae using PCR-RAPD. Available from Nature Precedings <http://hdl.handle.net/10101/npre.2008.2403.1> (2008)
- Version info:
-
Other versions of this document in Nature Precedings
None.
Other versions of this document elsewhere on the web
None known.
Dr. Dinesh Raj Modi on 16 October 2008 17:09 UTC
This a very good paper towards genetic variability study of Xantomonas mangiferae strains using RAPD marker, especially in the sense that it may be first report from india to generate a informative data.
From:
Dr. D.R. Modi (Professor), Head, Department of Biotechnology, BBA University, LKO, India
E-mail: drmodilko [at] gmail.com