1 vote

Introduction of Transmembrane Inner Ear (tmie) Gene Can Recover the Hearing Impairment and Abnormal Behavior in the Circling Mouse

Mi Jung Shin¹, Jeong-Han Lee², Dong Hoon Yu¹, Bong Soo Kim¹, Hei Jung Kim¹, Sung Hyun Kim¹, Myoung Ok Kim¹, Channy Park², Byung-Hwa Hyun³, Sanggyu Lee¹, Jun-Gyo Suh⁴, Hong-Seob So², Raekil Park² & Zae Young Ryoo¹

Correspondence: (Login to view email address)

1. Kyungpook National University, School of Life Sciences and Biotechnology
2. Wonkwang University College of Medicine, Vestibulocochlear Research Center
3. Korea Research Institute of Bioscience and Biotechnology, Disease Model Research Center
4. Hallym University, Department of Medical Genetics and Experimental Animal Center

PDF (721.3 KB)

Document Type:

Manuscript

Date:

Received 22 July 2008 07:13 UTC; Posted 28 July 2008

Subjects:

Developmental Biology, Genetics & Genomics, Neuroscience

Tags:

• ear
• circling mouse
• transmembrane inner ear
• transgenic animal
• hearing loss
• organ of Corti
• tmie
• mouse
• deafness

Abstract:

The spontaneous mutant circling mouse (cir/cir) shows a circling behavior and hearing loss. We produced transgenic mice overexpressing the causative gene, transmembrane inner ear (tmie), for the phenotypic rescue of the circling mouse. Through the continuous breeding with circling mice, the cir/cir homozygous mice carrying the transgene (cir/cir-tg) were produced. The rescued cir/cir -tg mice were able to swim in the water with proper orientation and did not show any circling behavior like wild type mice. Western blot and immunohistochemical analysis exhibited that the transgenic tmie was expressed in the inner ear. Inner and outer hair cells were recovered in the cochlea and spiral ganglion neurons were also recovered in the rescued mice. Auditory brainstem response (ABR) test demonstrated that the cir/cir -tg mice are able to respond to sound. This study demonstrates that tmie transgene can recover the hearing impairment and abnormal behavior in the circling mouse.

Discussion

Votes:

1 vote

(Login to vote)

Comments:

0 comments

(Login to post a comment)

Share:

(Login to share with a colleague)

Additional information

License:

This document is licensed to the public under the Creative Commons Attribution 3.0 License

How to cite this document:

Shin, Mi Jung, Lee, Jeong-Han, Yu, Dong Hoon, Kim, Bong Soo, Kim, Hei Jung, Kim, Sung Hyun, Kim, Myoung Ok, Park, Channy, Hyun, Byung-Hwa, Lee, Sanggyu, Suh, Jun-Gyo, So, Hong-Seob, Park, Raekil, and Ryoo, Zae Young. Introduction of Transmembrane Inner Ear (tmie) Gene Can Recover the Hearing Impairment and Abnormal Behavior in the Circling Mouse. Available from Nature Precedings <http://hdl.handle.net/10101/npre.2008.2106.1> (2008)

Version info:

Other versions of this document in Nature Precedings

None.

Other versions of this document elsewhere on the web

None known.

Submit a new version link