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The effects of anti-sense interleukin-5 gene transferred by recombinant adeno-associated virus in allergic rats

Daxiong Zeng¹, Yong Cao¹, Qingfeng Song¹, Chao Cao¹, Xiansheng Liu¹, Yongjian Xu¹ & Weining Xiong¹

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1. Department of Respiratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology

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Document Type:

Manuscript

Date:

Received 03 July 2008 06:19 UTC; Posted 09 July 2008

Subjects:

Biotechnology, Molecular Cell Biology

Tags:

• recombinant adeno-associated virus
• interleukin-5
• gene therapy
• asthma

Abstract:

The accumulation and infiltration of eosinophils in airways is one of the most important characteristics of asthma, and is mediated partly by secretion of IL-5 from Th2 lymphocytes. It is well known that interleukin-5 (IL-5) played an important role in the regulation of eosinophils. In this study, an anti-sense IL-5 gene transferred by recombinant adeno-associated virus (rAAV-ASIL-5) was prepared to transfect allergic rats. It was found that the expression of IL-5 protein in plasma and BALF were inhibited significantly. The rAAV-ASIL-5-mediated suppression of total cell counts in peripheral blood and BALF were also observed. Moreover, rAAV-ASIL-5 remarkably reduced the eosinophil counts in peripheral blood and BALF, as well as the expression of ECP protein in plasma and BALF. The inflammation in lungs of rAAV-ASIL-5 pretreated rats also became slighter when compared with allergic rats. Otherwise, no apparent pathological damage to vital organs of rats was found. In conclusion, recombinant adeno-associated virus-mediated delivery of anti-sense IL-5 gene inhibited the accumulation of eosinophils and the airways inflammation in rat model of allergic asthma via suppressing IL-5 expression. It suggested the feasibility of rAAV-ASIL-5 in the gene therapy for allergic asthma and other eosinophilic diseases.

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This document is licensed to the public under the Creative Commons Attribution 3.0 License

How to cite this document:

Zeng, Daxiong, Cao, Yong, Song, Qingfeng, Cao, Chao, Liu, Xiansheng, Xu, Yongjian, and Xiong, Weining. The effects of anti-sense interleukin-5 gene transferred by recombinant adeno-associated virus in allergic rats. Available from Nature Precedings <http://hdl.handle.net/10101/npre.2008.2036.1> (2008)

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