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Effects of Cations and PH on Antimicrobial Activity of Thanatin and s-Thanatin against Escherichia coli ATCC25922 and B. subtilis ATCC 21332

Guoqiu Wu¹, Jiaxuan Ding², Hui Li³, Linxian Li³, Rui Zhao², Xiaobo Fan² & Zilong Shen²

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1. Zhongda Hospital, Southeast University
2. Biotechnology Center, School of Life Science and Technology, China Pharmaceutical University
3. School of Pharmacy, China Pharmaceutical University

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Document Type:

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Date:

Received 25 June 2008 07:10 UTC; Posted 25 June 2008

Subjects:

Microbiology, Pharmacology

Tags:

• pH
• susceptibility tests
• cations
• antimicrobial peptides
• Thanatin
• s-Thanatin
• Bacteria

Abstract:

Thanatin and s-thanatin were insect antimicrobial peptides which have shown potent antimicrobial activities on a variety of microbes. In order to investigate the effect of cations and pH on the activity of these peptides against Gram-negative bacteria and Gram-positive bacteria, the antimicrobial activities of both peptides were studied in increasing concentrations of monovalent cations (K⁺ and Na⁺), divalent cations (Ca²⁺ and Mg²⁺) and H⁺. The NCCLS broth microdilution method showed that both peptides were sensitive to the presence of cations. The divalent cations showed more antagonized effect on the activity against Gram-negative bacteria than the monovalent cations, since the two peptides lost the ability to inhibit bacterial growth at a very low concentration. In addition, the activities of both peptides tested were not significantly affected by pH. Comparing to studies of other antibacterial peptide activities, our data support a hypothesis that positive ions affect the sensitivity to cation peptides.

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This document is licensed to the public under the Creative Commons Attribution 3.0 License

How to cite this document:

Wu, Guoqiu, Ding, Jiaxuan, Li, Hui, Li, Linxian, Zhao, Rui, Fan, Xiaobo, and Shen, Zilong. Effects of Cations and PH on Antimicrobial Activity of Thanatin and s-Thanatin against Escherichia coli ATCC25922 and B. subtilis ATCC 21332. Available from Nature Precedings <http://hdl.handle.net/10101/npre.2008.2006.1> (2008)

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