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Green Fluorescent Protein (GFP) in Vector Systems Played Sense Role of Epigenetic in Plants

Hany A. El-Shemy¹, Mutasim M. Khalafalla² & Masao Ishimoto³

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1. Biochemistry Department, Faculty of Agriculture, Cairo University, Giza 12613, Egypt
2. Commission for Biotechnology and Genetic Engineering, National Centre for Research P.O. Box 2404 Khartoum, Sudan
3. National Agricultural Research Center for Western Region, 6-12-1 Nishifukatsu, Fukuyama, Hiroshima 721-8514, Japan

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Document Type:

Manuscript

Date:

Received 29 April 2008 20:38 UTC; Posted 30 April 2008

Subjects:

Biotechnology, Molecular Cell Biology, Plant Biology

Tags:

• plant biotechnology
• GFP
• Epigenetics

Abstract:

The green fluorescent protein (GFP) of jellyfish (Aequorea victoria) has significant advantages over other reporter genes, because expression can be detected in living cells without any substrates. Recently, epigenetic phenomena are important to consider in plant biotechnology experiments for elucidate unknown mechanism. Therefore, soybean immature cotyledons were generated embryogenesis cells and engineered with two different gene constructs (pHV and pHVS) using gene gun method. Both constructs contain a gene conferring resistance to hygromycin (hpt) as a selective marker and a modified glycinin (11S globulin) gene (V3-1) as a target. However, sGFP(S65T) as a reporter gene was used only in pHVS as a reporter gene for study the relation between using sGFP(S65T) and gene silencing phenomena. Fluorescence microscopic was used for screening after the selection of hygromycin, identified clearly the expression of sGFP(S65T) in the transformed soybean embryos bombarded with the pHVS construct. Protein analysis was used to detect gene expression overall seeds using SDS-PAGE. Percentage of gene down regulation was highly in pHV construct compared with pHVS. Thus, sGFP(S65T) as a reporter gene in vector system may be play useful role for transgenic evaluation and avoid gene silencing in plants for the benefit of plant transformation system.

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This document is licensed to the public under the Creative Commons Attribution 3.0 License

How to cite this document:

El-Shemy, Hany, Khalafalla, Mutasim, and Ishimoto, Masao. Green Fluorescent Protein (GFP) in Vector Systems Played Sense Role of Epigenetic in Plants . Available from Nature Precedings <http://hdl.handle.net/10101/npre.2008.1845.1> (2008)

Version info:

Published version:

http://www.horizonpress.com/cimb/abstracts/v11/i21.html (Peer Reviewed) Published as 'The Role of Green Fluorescent Protein (GFP) in Transgenic Plants to Reduce Gene Silencing Phenomena' Curr. Issues Mol. Biol. 11 (Suppl. 1): i21-28.

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