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Barlow-Hall in vitro Evolution Protocol

Barry G. Hall¹ and Miriam Barlow²

Correspondence: (Login to view email address)

1. Bellingham Research Institute
2. University of California at Merced

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Document Type:

Manuscript

Date:

Received 07 April 2008 21:49 UTC; Posted 08 April 2008

Subjects:

Genetics & Genomics, Molecular Cell Biology, Evolutionary Biology

Tags:

• experimental prediction
• in vitro evolution
• method
• protocol

Abstract:

The Barlow-Hall method for in vitro evolution is a simple alternative to methods such as DNA shuffling and is particularly applicable to predicting the evolution of target genes in nature. This detailed protocol includes the procedure itself and, as Appendices, describes particularly effective protocols for electroporation and for preparation of electrocompetent cells.

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License:

This document is licensed to the public under the Creative Commons Attribution 3.0 License

How to cite this document:

Hall, Barry and Barlow, Miriam. Barlow-Hall in vitro Evolution Protocol. Available from Nature Precedings <http://hdl.handle.net/10101/npre.2008.1772.1> (2008)

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