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Azadirachta indica induced suppression of Mycobacterium tuberculosis secreted proteins in human monocyte

Jawed Iqbal¹, Ajay Kumar², & Najmul Islam¹

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1. Department of Biochemistry, Faculty of Medicine, J. N. Medical College, A.M.U., Aligarh 202002, U.P., India
2. Department of Biochemistry, G.V.S.M. Medical College, Kanpur, U. P, India.

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Document Type:

Poster

Date:

Received 01 February 2008 12:38 UTC; Posted 01 February 2008

Subjects:

Microbiology, Molecular Cell Biology

Tags:

• neem extract
• tuberculosis
• monocytes

Abstract:

The H37Rv strain of MTB was grown in modified Sauton’s medium till mid-log phase. Peripheral blood mononuclear cells (PBMC) were isolated by density gradient sedimentation on Ficoll-Paque separation medium. Adherent monocytes obtained from PBMC’s were infected with H37Rv for 90 min. in the ratio of 1:1 (1 bug: 1 cell). Thereafter, after washing, infected cells were co-cultured with varying doses of neem extract for 24 hrs, and harvested subsequently. Modulation of secreted TNF-α, iNOS and MTB Ag85 complex expressions in culture supernatants was estimated by ELISA.
We report the high basal expression of secreted tumor necrosis factor-α (TNF-α), inducible nitric oxide synthase (iNOS) and MTB Ag85 complex in MTB-infected monocytes was suppressed by neem extract in a dose-dependent manner (P<0.001) in the expressions of
secreted TNF-α, iNOS and MTB Ag85 respectively in 24 hr culture supernatants of MTB-infected monocytes.
Thus, neem extract seems to be a potential future adjunct for in-depth studies in the management of tuberculosis.

Presented at:

34th Annual Conference of Clinical Biochemists of India , 18 December 2007

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This document is licensed to the public under the Creative Commons Attribution 3.0 License

How to cite this document:

Iqbal, Jawed, Kumar, Ajay, and Islam, Najmul. Azadirachta indica induced suppression of Mycobacterium tuberculosis secreted proteins in human monocyte. Available from Nature Precedings <http://dx.doi.org/10.1038/npre.2008.1562.1> (2008)

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