Document information
Beneficial role of allicin from garlic in cervical cancer
Correspondence: (Login to view email address)
- Department of Biochemistry, Faculty of Medicine, J. N. Medical College, A.M.U., Aligarh 202002, U.P., India
- Document Type:
- Poster
- Date:
- Received 01 February 2008 12:21 UTC; Posted 01 February 2008
- Subjects:
- Cancer, Molecular Cell Biology
- Abstract:
Introduction: Cervical cancer remains a global health concern for females. Thus, in order to control cervical cancer, attempts are being made by researchers globally to somehow induce programmed cell death in the said cancerous cells. Wide spectrums of molecules are being probed for its ability to induce apoptosis in cervical cancer cells. Focus has now shifted in exploring natural compounds having antioxidant and anti-inflammatory molecules that may induce apoptosis in cancerous cells. Thus, we have employed allicin from garlic- a natural antioxidant, to probe the above in the present study.
Objective: To probe whether or not allicin from garlic, a natural antioxidant, induces apoptosis in monocytes from patients with cervical cancer.
Results: Allicin (500 ng/ml) reduced cell viability to 27% after 24 hours of treatment. Moreover, allicin-induced apoptosis was ascertained by measuring the activity of caspase-3, caspase-8 and caspase-9-like proteases in allicin treated and untreated monocytes from cervical cancer patients. Monocyte co-cultured with allicin for 24 hrs exhibited higher activity of caspase-3 followed by caspase-8 and caspase-9 like proteases, thereby indicating that the activation of caspase-3 like proteases was associated with reduced cell survival and apoptotic death of allicin-treated cervical cancer monocytes. This was ascertained by pre-treatment of cancer cells with cell permeable inhibitor Z-VAD-FMK (caspase-3 inhibitor), Z-IETD-FMK (caspase-8 inhibitor) and Z-LEHD-FMK (caspase-9 inhibitor) followed by allicin for 24 hrs (p<0.001).
Conclusion: Allicin from garlic may act as an adjunct in the chemotherapy of cervical cancer.- Presented at:
- 34th Annual Conference of Clinical Biochemists of India , 18 December 2007
Discussion
- Votes:
-
4 votes
- Comments:
-
0 comments
- (Login to share with a colleague)
Additional information
- License:
- This document is licensed to the public under the Creative Commons Attribution 3.0 License
- How to cite this document:
-
Ansari, Irfan Ahmad and Islam, Najmul . Beneficial role of allicin from garlic in cervical cancer. Available from Nature Precedings <http://dx.doi.org/10.1038/npre.2008.1561.1> (2008)
- Version info:
-
Other versions of this document in Nature Precedings
None.
Other versions of this document elsewhere on the web
None known.