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Allicin from garlic suppresses TNF-α and augments IFN-γ expressions in monocyte cultures from patients with Vaginitis
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- Department of Biochemistry, Faculty of Medicine, J. N. Medical College, A.M.U., Aligarh 202002, U.P., India
- Department of Biochemistry, King George Medical University, Lucknow, U.P., India
- Department of Obstetrics and Gynecology, Faculty of Medicine, J. N. Medical College, A.M.U., Aligarh 202002, U.P., India
- Department of Biochemistry, ERA'S Medical College and Hospital, Lucknow, U.P., India
- Document Type:
- Manuscript
- Date:
- Received 01 February 2008 09:49 UTC; Posted 01 February 2008
- Subjects:
- Immunology, Microbiology, Molecular Cell Biology
- Abstract:
Objective: We tried to explore beneficial effects of allicin- a natural antioxidant from garlic in the possible management of vaginitis.
Methods: Peripheral blood mononuclear cells (PBMC’s) were isolated from blood of patients having vaginal infections as well as normal healthy subjects as per our published protocol. Monocytes (MN’s) from above PBMC’s were adhered, rested overnight and cultured without or with varying doses of allicin (0-500 ng/ml). Some cultures received SN50 and SN50M (100 ug/ml). After 24 hrs cultures, the cells were harvested and the supernatants subjected to secreted TNF-α and sIFN-γ assays by ELISA. Treated/untreated harvested cells were subjected to glutathione peroxidase (GPx) activity assay.
Results: We show involvement of inflammatory cytokines and reactive oxygen species (ROS) in vaginitis. Augmented secreted TNF-α and decreased sIFN-γ levels were observed in monocyte cultures of vaginal patients. Relative to healthy controls, a decrease by 2.5-fold in glutathione peroxidase (GPx) activity in cultures of vaginal patients was observed. Treatment with allicin from garlic appreciably decreased secreted TNF-α with simultaneous amelioration in sIFN-γ and GPx activity. Allicin (500 ng/ml) ameliorated GPx activity by ~ 2.16-folds in patient monocyte cultures. The anti-oxidant and anti-inflammatory actions of allicin induced in monocyte cultures of vaginal infection patients were mediated via NF-κB.
Conclusion: Thus, the above results may help in understanding the use of allicin as an adjunct in vaginal infection therapy, and in turn, may address the globally female health.
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- This document is licensed to the public under the Creative Commons Attribution 3.0 License
- How to cite this document:
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Islam, Najmul, Iqbal, Jawed, Hasan, Nazarul, Fatima, Zeeshan, Thakur, Hamida, Hasan, Nazia, Mahdi, Abbas Ali, Khan, Tamkin, Bano, Farzana, and Ansari, Irfan Ahmad. Allicin from garlic suppresses TNF-α and augments IFN-γ expressions in monocyte cultures from patients with Vaginitis. Available from Nature Precedings <http://dx.doi.org/10.1038/npre.2008.1560.1> (2008)
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