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Interleukin-10 containing normal human serum inhibits granzyme B release but not perforin release from alloreactive and EBV-specific T cell clones

Motoko Nishimura¹, Hideya Sato², Hitoshi Okazaki², Masahiro Satake³, & Kenji Tadokoro²

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1. Japanese Red Cross Society, Research and Development
2. Japanese Red Cross Society, Reserach and Development
3. Tokyo Metropolitan Red Cross Blood Center, Research

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Document Type:

Manuscript

Date:

Received 05 December 2007 07:51 UTC; Posted 07 December 2007

Subjects:

Immunology

Tags:

• T cells
• interleukin-10
• cytokine
• inflammation
• immunoregulation

Abstract:

Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor, has pleiotropic effects in immunoregulation and inflammation. It is capable of inhibiting synthesis of pro-inflammatory cytokines like interferon γ (IFNγ), IL-2, IL-3, tumor necrosis factor α (TNFα) and granulocyte macrophage colony stimulating factor (GM-CSF) made by cells such as macrophages and T helper Type 1 cells. We observed that normal human serum, derived from a healthy individual but containing large amounts of IL-10 (arbitrarily designated as “IL-10 serum”), inhibited cytotoxic activity and interfered with granzyme B release from alloreactive cytotoxic T cell clones in vitro, but did not affect perforin release. The addition of normal human serum containing high levels of anti-IL-10 IgG (arbitrarily designated as “anti-IL-10 IgG serum”) neutralized the inhibitory effects of IL-10 serum. Moreover, we have identified that cytotoxic activity and granzyme B release from an Epstein-Barr virus (EBV)-specific cytotoxic T cell clone was similarly inhibited in the presence of IL-10 serum, while perforin release was unaffected. Anti-IL-10 IgG serum also appeared to neutralize the inhibitory effect of IL-10 serum on an EBV-specific T cell clone. When anti-IL-10 IgG was depleted from anti-IL-10 IgG containing serum (arbitrarily designated as “anti-IL10 IgG free serum”), the neutralizing effect disappeared for both alloreactive and an EBV-specific T cell clone.

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This document is licensed to the public under the Creative Commons Attribution 3.0 License

How to cite this document:

Nishimura, Motoko, Sato, Hideya, Okazaki, Hitoshi, Satake, Masahiro, and Tadokoro, Kenji. Interleukin-10 containing normal human serum inhibits granzyme B release but not perforin release from alloreactive and EBV-specific T cell clones. Available from Nature Precedings <http://hdl.handle.net/10101/npre.2007.1390.1> (2007)

Version info:

Published version:

10.1016/j.cellimm.2008.03.001 (Peer Reviewed) Published in: Cellular Immunology, Volume 251, Issue 1, 2008, Pages 31-36.

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