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doi:10.1038/npre.2007.1238.1
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Collagen-bound low density lipoprotein modifies endothelial cell adhesion to type V collagen: Implications for atherosclerosis

Stefan Lorkowski1, Jürgen Rauterberg1, Bärbel Harrach-Ruprecht1, & David Troyer1

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  1. Leibniz Institute of Arteriosclerosis Research
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Manuscript
Date:
Received 18 October 2007 16:32 UTC; Posted 18 October 2007
Subjects:
Molecular Cell Biology
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Abstract:

Low density lipoprotein (LDL) is retained in the extracellular matrix of the arterial wall where it is considered to be atherogenic, but little is known about how cell adhesion to the matrix is affected by collagen-bound LDL. We tested the effect of native, oxidized and acetylated LDL reacted with adsorbed monomeric type I, III and V collagen on endothelial cell adhesion to collagen using a colorimetric adhesion assay. We found that none of the LDL species affected adhesion to type I and III collagen, but that collagen-bound native and acetylated LDL enhanced attachment to type V collagen, whereas bound oxidized LDL inhibited adhesion to this collagen. We suggest that oxidized LDL associated with type V collagen in the arterial wall would favor de-endothelialization and contribute to atherogenesis and thrombosis.

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This document is licensed to the public under the Creative Commons Attribution 2.5 License
How to cite this document:

Lorkowski, Stefan, Rauterberg, Jürgen, Harrach-Ruprecht, Bärbel, and Troyer, David. Collagen-bound low density lipoprotein modifies endothelial cell adhesion to type V collagen: Implications for atherosclerosis. Available from Nature Precedings <http://dx.doi.org/10.1038/npre.2007.1238.1> (2007)

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