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A database of naturally occurring human urinary peptides and proteins for use in clinical applications
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- Mosaiques diagnostics & therapeutics AG
- University of Wisconsin-Madison, Department of Chemistry and Biomolecular Chemistry
- Ludwig-Maximilians-University Munich
- Hannover Medical School, Department of Clinical Immunology
- University of Glasgow, BHF Glasgow Cardiovascular Research Centre
- CHU de Toulouse, Pediatric Nephrology Unit
- Hannover Medical School, Children's Hospital
- Hannover Medical School, Department of Nephrology
- GSF - National Research Centre for Environment and Health, Institute for Ecological Chemistry
- Hannover Medical School, Department of Hematology, Hemostasis and Oncology
- University of Glasgow, Department of Computing Science
- University of Wisconsin-Madison, Department of Chemistry
- University Duisburg-Essen, Department of Nephrology
- University Hospital Hamburg-Eppendorf, Department of Psychiatry
- University of Melbourne, Austin Health & Northern Health, Department of Medicine
- University of Alabama at Birmingham
- Thermo Fisher Scientific GmbH
- Saxony Centre for Nephrology, Transplantation Centre, Department of Nephrology Lower
- University of Glasgow, The Beatson Institute for Cancer Research & Sir Henry Wellcome Functional Genomics Facility
- Harvard Medical School, Department of Medicine
- University of Modena and Reggio Emilia, Department of Oncology and Hematology
- UPJV, INSERM, ERI-12, and Amiens University Hospital
- University of Regensburg, Children’s Hospital
- University of Aalen
- University of Alabama at Birmingham, Microbiology
- Heart Research Institute, Centre for Thrombosis & Myocardial Infarction
- Steno Diabetes Centre
- Clinical Centre Bayreuth, Department of Nephrology
- University of Leipzig, Department of Diagnostic Radiology
- University of Virginia
- Mahidol University, Faculty of Medicine at Siriraj Hospital
- University Hospital of Ghent, Department of Internal Medicine
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- Manuscript
- Date:
- Received 11 October 2007 07:09 UTC; Posted 12 October 2007
- Subjects:
- Biotechnology
- Abstract:
Owing to its availability, ease of collection and correlation with (patho-) physiology, urine is an attractive source for clinical proteomics. However, the lack of comparable datasets from large cohorts has greatly hindered development in this field. Here we report the establishment of a high resolution proteome database of naturally occurring human urinary peptides and proteins – ranging from 800-17,000 Da – from over 3,600 individual samples using capillary electrophoresis coupled to mass spectrometry, yielding an average of 1,500 peptides per sample. All processed data were deposited in an SQL database, currently containing 5,010 relevant unique urinary peptides that serve as classifiers for diagnosis and monitoring of diseases, including kidney and vascular diseases. Of these, 352 have been sequenced to date. To demonstrate the applicability of this database, two examples of disease diagnosis were provided: For renal damage diagnosis, patients with a specific renal disease were identified with high specificity and sensitivity in a blinded cohort of 131 individuals. We further show definition of biomarkers specific for immunosuppression and complications after transplantation (Kaposi’s sarcoma). Due to its high information content, this database will be a powerful tool for the validation of biomarkers for both renal and non-renal diseases.
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- This document is licensed to the public under the Creative Commons Attribution 2.5 License
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Zürbig, Petra, Coon, Joshua, Bauer, Hartwig, Behrens, Georg, Dakna, Mohammed, Dominiczak, Anna, Decramer, Stephane, Ehrich, Jochen, Fliser, Danilo, Frommberger, Moritz, Ganser, Arnold, Giolami, Mark, Golovko, Igor, Good, David, Gwinner, Wilfried, Haubitz, Marion, Herget-Rosenthal, Stefan, Jahn, Holger, Jerums, George, Julian, Bruce, Kellmann, Markus, Kliem, Volker, Kolch, Walter, Krolewski, Andrzej, Luppi, Mario, Massy, Ziad, Melter, Michael, Neusüss, Christian, Novak, Jan, Peter, Karlheinz, Rossing, Kasper, Rupprecht, Harald, Schanstra, Joost, Schiffer, Eric, Stolzenburg, Jens-Uwe, Tarnow, Lise, Theodorescu, Dan, Thongboonkerd, Visith, Vanholder, Raymond, Weissinger, Eva, Mischak, Harald, and Schmitt-Kopplin, Philippe. A database of naturally occurring human urinary peptides and proteins for use in clinical applications. Available from Nature Precedings <http://hdl.handle.net/10101/npre.2007.1219.1> (2007)
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