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Upstream Stimulatory Factor (USF) and CCAAT/Enhancer Binding Protein δ (C/EBPδ) Compete for overlapping Sites in the Negative Regulatory Region of the HIV-1 LTR

Torik T. A. Ayoubi¹, Sandra M. P. Meulemans² & Wim W. J. M. Van de Ven²

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1. Genome Center Maastricht, Department of Genetics and Cell Biology, Universiteitssingel 50, 6229 ER Maastricht, The Netherlands
2. Laboratory for Molecular Oncology, Center for Human Genetics, University of Leuven, Herestraat 49, B-3000 Leuven, Belgium

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Document Type:

Manuscript

Date:

Received 08 October 2007 15:49 UTC; Posted 09 October 2007

Subjects:

Immunology, Molecular Cell Biology

Tags:

• gene expression
• transcription
• USF1
• HIV-1
• LTR
• CEBP

Abstract:

Human immunodeficiency virus type 1 (HIV-1) is a human retrovirus and the causative agent of the acquired immunodeficiency syndrome. Genetic analysis has revealed that the HIV-1 LTR contains a potential negative regulatory element (NRE) with an E box, the recognition sequence for the helix-loop-helix (HLH) family of transcription factors. Furthermore, the upstream stimulatory factor (USF) has been implicated as a negative regulator of HIV-1 expression. Here, we report that the NRE is a composite element and that both C/EBPδ and USF can specifically bind to the NRE. The recognition sequence for C/EBPδ overlaps with the E box in the NRE of HIV-1. Competition experiments showed that either USF or C/EBPδ binds to this NRE but not both together.

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This document is licensed to the public under the Creative Commons Attribution 2.5 License

How to cite this document:

Ayoubi, Torik, Meulemans, Sandra, and Van de Ven, Wim. Upstream Stimulatory Factor (USF) and CCAAT/Enhancer Binding Protein δ (C/EBPδ) Compete for overlapping Sites in the Negative Regulatory Region of the HIV-1 LTR. Available from Nature Precedings <http://dx.doi.org/10.1038/npre.2007.1212.1> (2007)

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