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Differential Gene Expression of Human Mast cell Activation Reveals Gene profiles of Innate and Adaptive Immunity.

Jayapal J. Manikandan¹, Reghunathan Renji¹, Peter Natesan Pushparaj¹, & Alirio Jose Melendez¹

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1. Department of Physiology, National University of Singapore

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Document Type:

Poster

Date:

Received 27 September 2007 14:37 UTC; Posted 28 September 2007

Subjects:

Immunology, Molecular Cell Biology, Pharmacology, Bioinformatics

Tags:

• gene expression profile
• innate immunity
• adaptive immunity
• mast cells
• oligonucleotide microarray

Abstract:

High-density oligonucleotide microarray is a promising approach for high throughput analysis. It has been extensively used in many areas of biomedical research. Immunoglobulin E (IgE) mediated allergic response (type-1 hypersensitivity) is one of the most powerful reactions of the immune system. Tissue Mast Cells (MCs) and circulating basophils are the major effector cells in these reactions. By dissecting the regulatory circuitry of mast cells by analyzing the genome wide effects of antigen stimulation triggered by FcεRI, offers a potential for finding novel genes as ‘targets’ for therapeutic intervention. In this work, we tried to study the gene expression pattern in IgE sensitized and FcεRI cross linked cord blood derived MCs using one of the latest techniques, high density oligonucleotide expression probe array (HG-Focus array, Gene Chip, Affymetrix, Santa Clara, CA). Microarray hybridization of RNA from cord blood derived MCs revealed coordinated changes in gene expression in response to IgE stimulation and receptor cross linking at different time points. Among the most prominent findings, we observed 2 to 32-fold increased expression of different transcripts. Real-time PCR confirmed reliability of microarray data. This enabled us to classify and cluster genes by functional families as well as to understand known genes in signaling pathways. These results defined a list of primary candidates for finding novel genes as ‘targets’ for therapeutic intervention.

Presented at:

International Conference on Analysis of Genomic Data, Harvard Medical School, Boston, MA, 10 May 2004

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This document is licensed to the public under the Creative Commons Attribution 2.5 License

How to cite this document:

Manikandan, Jayapal, Renji, Reghunathan, Pushparaj, Peter Natesan, and Melendez, Alirio Jose. Differential Gene Expression of Human Mast cell Activation Reveals Gene profiles of Innate and Adaptive Immunity. . Available from Nature Precedings <http://dx.doi.org/10.1038/npre.2007.1148.1> (2007)

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